Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amiodarone-associated changes in human neutrophils.

P Somani, V A Simon, P N Temesy-Armos

    The American Journal of Cardiology
    |March 1, 1986
    PubMed
    Summary

    White blood cell (WBC) concentrations of amiodarone and its metabolite, desethylamiodarone, correlate with tissue levels and may help monitor patients. Electron microscopy revealed inclusion bodies in neutrophils of patients on amiodarone therapy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Wide-area transepithelial sampling with computer-assisted 3-dimensional analysis (WATS) markedly improves detection of esophageal dysplasia and Barrett's esophagus: analysis from a prospective multicenter community-based study.

    Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2018
    Same author

    Neonatal sepsis in a tertiary care center in central India: microbiological profile, antimicrobial sensitivity pattern and outcome.

    Journal of neonatal-perinatal medicine·2013
    Same author

    An evidence-based analysis of epidemiologic associations between lymphatic and hematopoietic cancers and occupational exposure to gasoline.

    Human & experimental toxicology·2013
    Same author

    Innervation of the extensor carpi radialis brevis and supinator muscles: Levels of origin and penetration of these muscular branches from the posterior interosseous nerve.

    Journal of shoulder and elbow surgery·2012
    Same author

    Rectal fistula due to ovarian teratoma.

    Endoscopy·2012
    Same author

    A comparison of high definition-image enhanced colonoscopy and standard white-light colonoscopy for colorectal polyp detection.

    Endoscopy·2011

    Area of Science:

    • Pharmacology
    • Cell Biology
    • Clinical Medicine

    Background:

    • Amiodarone is an antiarrhythmic drug with a long half-life.
    • Its distribution and accumulation in tissues are not fully understood.
    • Monitoring drug levels in accessible cells like white blood cells (WBCs) could offer insights.

    Purpose of the Study:

    • To measure amiodarone and desethylamiodarone concentrations in plasma and blood cells (WBCs, RBCs) of patients on chronic therapy.
    • To investigate the correlation between plasma and cellular drug levels.
    • To examine ultrastructural changes in neutrophils and relate them to drug accumulation.

    Main Methods:

    • Plasma, WBC, and RBC samples were collected from 14 patients on amiodarone.
    • Drug concentrations were quantified using unspecified methods.

    Related Experiment Videos

  • Neutrophils underwent electron microscopic examination.
  • Tissue samples from one patient with neuropathy were analyzed.

    • Main Results:

    • Mean plasma concentrations of amiodarone and desethylamiodarone were 2.4 and 1.6 µg/ml, respectively.
    • WBC concentrations of amiodarone and desethylamiodarone were significantly higher during maintenance than loading phases.
    • No linear correlation was found between WBC and plasma drug levels.
    • High amiodarone and desethylamiodarone concentrations were found in nerve and muscle tissue of a patient with neuropathy.
    • All patients' neutrophils exhibited characteristic 'onionoid bodies' on electron microscopy.

    Conclusions:

    • WBC drug concentrations may reflect tissue drug levels.
    • Electron microscopic changes in neutrophils could serve as a biomarker for amiodarone accumulation.
    • These findings suggest potential methods for monitoring amiodarone therapy and patient outcomes.