LINC01764 promotes colorectal cancer cells proliferation, metastasis, and 5-fluorouracil resistance by regulating glucose and glutamine metabolism via promoting c-MYC translation
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View abstract on PubMed
Summary
This summary is machine-generated.High LINC01764 long noncoding RNA expression predicts poor colorectal cancer (CRC) outcomes and 5-fluorouracil resistance. It promotes CRC progression by enhancing metabolism and c-MYC translation via hnRNPK, suggesting it as a prognostic biomarker.
Area Of Science
- Oncology
- Molecular Biology
- Genetics
Background
- Biomarkers for predicting chemotherapeutic response and prognosis in colorectal cancer (CRC) are limited.
- Long noncoding RNAs (lncRNAs) play crucial roles in CRC development and progression.
- Identifying novel lncRNAs may offer predictive potential for CRC treatment outcomes.
Purpose Of The Study
- To investigate the role of LINC01764 in colorectal cancer (CRC) progression and response to chemotherapy.
- To elucidate the molecular mechanisms underlying LINC01764's function in CRC.
- To assess LINC01764's potential as a biomarker for CRC prognosis and chemotherapy response.
Main Methods
- Analysis of LINC01764 expression in CRC patient datasets (Fudan University Shanghai Cancer Center, TCGA).
- Gene set enrichment analysis to identify associated pathways.
- In vitro experiments including cotransfection, RNA pulldown, RIP, protein synthesis assays, and dual-luciferase reporter assays to determine molecular interactions.
Main Results
- High LINC01764 expression is correlated with CRC metastasis, poor response to FOLFOX/XELOX chemotherapy, and adverse prognosis.
- LINC01764 promotes CRC cell proliferation and metastasis by enhancing glycolysis and glutamine metabolism.
- LINC01764 induces 5-fluorouracil (5-FU) resistance by upregulating c-MYC translation via hnRNPK and downregulating UPP1.
Conclusions
- LINC01764 promotes colorectal cancer progression and 5-FU chemoresistance through hnRNPK-mediated c-MYC internal ribosome entry site (IRES)-dependent translational regulation.
- LINC01764's oncogenic effects involve modulation of glucose and glutamine metabolism.
- LINC01764 represents a potential predictive biomarker for chemotherapy response and prognosis in colorectal cancer.
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