Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

3.2K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.2K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.6K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.6K
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

6.6K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRANSFORM-1 Phase 3 study: Efficacy and safety of navitoclax plus ruxolitinib in patients with untreated myelofibrosis.

Blood·2026
Same author

Type I interferon-activated NK cells control polycythemia vera in vivo.

Blood·2026
Same author

CAR T-cells targeting CD117 effectively eliminate mast cells in preclinical models of advanced systemic mastocytosis.

Leukemia·2026
Same author

The lysine-specific demethylase 1 (LSD1) inhibitor bomedemstat in myelofibrosis: results from a phase 1/2 study.

Blood advances·2026
Same author

Allogeneic Immune Cell Perfusion Inhibits the Growth of Vascularized 3D In Vitro Tumor Models, Induces Vascular Regression and Desmoplasia, but Promotes Tumor Cell Invasion.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Frequent and clinically relevant germline DNA repair gene variants in young and familial myeloproliferative neoplasms.

Blood cancer journal·2026

Related Experiment Video

Updated: Jun 7, 2025

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.3K

Cell Adhesion and Local Cytokine Control on Protein-Functionalized PNIPAM-co-AAc Hydrogel Microcarriers.

Sebastian Bernhard Rauer1,2, Lucas Stüwe1, Lea Steinbeck1

  • 1Chemical Process Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074, Aachen, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|November 13, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed tunable hydrogel microcarriers from poly(N-isopropylacrylamide) and acrylic acid for enhanced cell expansion. These adaptable biomaterials support cell growth and protein delivery in bioreactors, addressing key challenges in biotechnological processes.

Keywords:
PNIPAMcell expansionmicrocarrierprotein functionalizationstimuli‐responsive hydrogel

More Related Videos

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

5.6K
Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

11.8K

Related Experiment Videos

Last Updated: Jun 7, 2025

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
11:31

Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues

Published on: August 28, 2014

13.3K
Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Published on: January 29, 2022

5.6K
Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering
12:22

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

11.8K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Biotechnology

Background:

  • Achieving high cell densities is crucial for economic biotechnological and biomedical processes.
  • Current microcarriers have limitations in mechanical properties and adaptability.
  • Tailorable microcarriers are needed to optimize cell phenotype, differentiation, and genetic stability.

Purpose of the Study:

  • To introduce tunable hydrogel microcarriers for advanced cell expansion.
  • To investigate the impact of microcarrier properties on cell-particle interactions.
  • To demonstrate the utility of these microcarriers in static and dynamic cell culture systems.

Main Methods:

  • Co-polymerization of poly(N-isopropylacrylamide) (PNIPAM) and acrylic acid (AAc) to create hydrogel microcarriers.
  • Characterization of microcarrier properties including stiffness and charge.
  • Cultivation of L929 mouse fibroblast cells and 32D myeloblast-like cells on microcarriers.
  • Assessment of cell attachment, growth, and cytokine delivery.

Main Results:

  • PNIPAM-co-AAc microcarriers exhibit adjustable matrix-like softness and adaptable gel charge.
  • Functional carboxyl groups enable electrostatic and covalent protein coupling.
  • Successful attachment and growth of L929 cells in static and stirred-tank bioreactor (STBR) cultivations.
  • Sustained release of interleukin-3 to 32D cells over 20 days.
  • Demonstrated ability to provide both covalently coupled and diffusively released cytokines.

Conclusions:

  • PNIPAM-co-AAc microcarriers offer a versatile platform for cell expansion with tunable properties.
  • These microcarriers can be functionalized for specific cell adhesion and growth factor delivery.
  • The developed system shows significant potential for improving biotechnological and biomedical applications requiring high cell densities.