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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.

Anneliese M M Gest¹, Ayse Z Sahan^1,2, Yanghao Zhong¹

¹Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.

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Summary

Fluorescent biosensors are crucial tools for studying cell signaling pathways in living systems. This review guides users through developing and applying these sensors for cell fate research.

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Area of Science:

Cell Biology
Biochemistry
Molecular Imaging

Background:

Cellular functions rely on complex signaling networks that dictate cell fate.
Fluorescent biosensors are essential for visualizing these dynamic signaling pathways in real-time within living cells.
The development of fluorescent biosensors has rapidly expanded, offering diverse targets, spectral options, and imaging compatibility.

Purpose of the Study:

To provide a comprehensive overview of fluorescent biosensors for studying cellular signaling pathways.
To guide researchers in selecting and developing fluorescent biosensors for specific applications.
To highlight recent advancements and applications of fluorescent biosensors in living systems.

Main Methods:

Review of existing literature on fluorescent biosensors and their applications.
Categorization of biosensors based on their targets and design principles.
Discussion of key considerations for fluorescent protein selection and smart tagging strategies.

Main Results:

An extensive landscape of fluorescent biosensors is now available for numerous cellular targets.
Biosensors are designed using various fluorescent proteins and sophisticated tagging strategies.
Recent applications demonstrate the utility of these sensors across diverse biological questions.

Conclusions:

Fluorescent biosensors are indispensable tools for dissecting complex cell signaling networks.
Careful consideration of biosensor design, fluorescent protein properties, and tagging is critical for successful application.
Continued innovation in biosensor technology promises deeper insights into cell fate determination.