Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neuromuscular dysfunction in patient-derived FUS<sup>R244RR</sup>-ALS iPSC model via axonal downregulation of neuromuscular junction proteins.

NAR molecular medicine·2025

Same author

RNA-binding fluorogenic probes: G-clamp conjugated with a thiazole orange derivative for screening RNA-binding small molecules.

RSC chemical biology·2025

Same author

Mechanistic insights into the basis of widespread RNA localization.

Nature cell biology·2024

Same author

mRNA stability and m<sup>6</sup>A are major determinants of subcellular mRNA localization in neurons.

Molecular cell·2023

Same author

Massively parallel identification of mRNA localization elements in primary cortical neurons.

Nature neuroscience·2023

Same author

The hnRNP C tetramer binds to CBC on mRNA and impedes PHAX recruitment for the classification of RNA polymerase II transcripts.

Nucleic acids research·2023

Search research articles

Related Experiment Video

Updated: Jun 7, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Concurrent Profiling of Localized Transcriptome and RNA Dynamics in Neurons by Spatial SLAMseq.

Sayaka Dantsuji¹, Marina Chekulaeva²

¹Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2024

Summary

This study introduces spatial SLAMseq, a new method to measure mRNA half-lives in specific neuronal compartments. This technique helps understand how RNA localization impacts neuronal function and development.

Keywords:

High-throughput sequencing Local translation Neuron SLAMseq mRNA localization mRNA stability

More Related Videos

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Single-cell Profiling of Developing and Mature Retinal Neurons

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

Related Experiment Videos

Last Updated: Jun 7, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing DIVA-Seq

Published on: October 26, 2018

Single-cell Profiling of Developing and Mature Retinal Neurons

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

Area of Science:

Molecular Biology
Neuroscience
Cell Biology

Background:

Asymmetric RNA distribution is crucial for cellular function and neuronal plasticity.
Understanding RNA localization mechanisms requires methods to analyze transcripts in specific cellular locations.
Existing methods have limitations in analyzing mRNA dynamics within subcellular compartments.

Purpose of the Study:

To develop and optimize a method for measuring mRNA half-lives in distinct subcellular neuronal compartments.
To enable concurrent analysis of mRNA dynamics and steady-state levels within neurons.
To provide a tool for investigating the role of RNA localization in neuronal function.

Main Methods:

Spatial SLAMseq combines SLAMseq technology with subcellular fractionation techniques.
The method allows for the separation of neuronal cell bodies into nuclear and cytoplasmic fractions.
It also enables analysis of mRNA in neurites, distinct cellular extensions of neurons.

Main Results:

Spatial SLAMseq successfully measures mRNA half-lives in neurites, and cytoplasmic and nuclear fractions.
The method provides insights into the dynamics and steady-state levels of localized transcripts.
This technique facilitates a deeper understanding of RNA regulation in neurons.

Conclusions:

Spatial SLAMseq is an optimized method for analyzing mRNA dynamics in subcellular neuronal compartments.
This technique is valuable for studying RNA localization's role in neuronal growth, plasticity, and information processing.
The method advances the study of molecular mechanisms underlying neuronal complexity.