Related Experiment Video
Updated: Jun 7, 2025
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
The impact of antioxidants on antioxidant capacity, DNA fragmentation, and chromatin quality in subfertile men: a
Mesut Sengul1, Neslihan Hekim2,3, Ramazan Asci1,3
1Ondokuz Mayıs University, Faculty of Medicine, Department of Urology - Samsun, Turkey.
Objective:
This randomized clinical trial study aims to investigate the effects of antioxidant food supplementation on the total antioxidant capacity of seminal plasma, sperm DNA fragmentation, sperm chromatin quality, and semen parameters.
Methods:
In this study, a total of 48 subfertile men with moderate physical activity were included. Group 1 was recommended to use the antioxidant supplements, while antioxidant food supplements were not given to Group 2. Total antioxidant capacity, sperm DNA fragmentation, sperm chromatin structure, hormone levels, physical activities, and semen parameters were evaluated before and after treatment. Total antioxidant capacity, sperm DNA fragmentation, and sperm chromatin structure were assessed using ELISA, transferase dUTP nick end labeling, and aniline blue staining, respectively.
Results:
Sperm DNA fragmentation (p=0.003) and histone/protamine ratio (p<0.001) were significantly decreased in the patients receiving antioxidant treatment. There was no statistical difference in the total antioxidant capacity values of the post-treatment groups.
Conclusion:
Antioxidant therapy seems to improve sperm DNA fragmentation and histone/protamine ratios in subfertile patients.
Clinical Trial Registration Number:
NCT06042738.
More Related Videos
10:24Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
