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Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes

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Designable Electrochemiluminescence Patterning for Renewable and Enhanced Bioimaging.

Xiaodan Gou^1,2, Zejing Xing¹, Zhichen Zhang³

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 210023, Nanjing, P. R. China.

Angewandte Chemie (International Ed. in English)

|November 13, 2024

Summary

Laser-induced photothermal effects restore electrode activity in electrochemical imaging, enhancing single biological entity visualization and bioassay sensitivity. This method regenerates active sites, overcoming passivation for improved stability and pattern control.

Keywords:

bioimaging electrochemiluminescence in situ regeneration passivation photothermal effect

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A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

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Published on: March 9, 2017

Area of Science:

Electrochemistry
Surface Science
Bioimaging

Background:

Electrochemical imaging offers detailed analysis of interface heterogeneity and reaction kinetics at the single-entity level.
Electrode passivation during electrochemical reactions leads to reduced active sites and compromised long-term stability, limiting applications.

Purpose of the Study:

To introduce a novel method utilizing laser-induced photothermal effects to restore electrochemical activity and overcome electrode passivation.
To demonstrate the application of this method for enhancing electrochemiluminescence (ECL) microscopy of single biological entities and bioassays.

Main Methods:

Employing laser-induced photothermal effects to regenerate active sites on electrode surfaces.
Utilizing co-localization characterization and X-ray photoelectron spectroscopy (XPS) to validate the mechanism of active site regeneration.
Investigating the surface-confined and voltage-dependent features of ECL for pattern manipulation.

Main Results:

Laser irradiation effectively removed the oxide film, restoring local surface ECL reactivity and enhancing micrometric patterns.
Improved image quality and distinct contour visualization of single biological entities, including bacteria and cells.
Enhanced ECL signal in bead-based bioassays due to the renewable electrode interface.

Conclusions:

Laser-induced photothermal effects provide a viable strategy to overcome electrode passivation in electrochemical applications.
The method enables precise micron pattern fabrication, erasure, and rewriting with good reversibility, showing anti-counterfeiting potential.
This approach significantly enhances sensitivity in electrochemical immunoassays and bioimaging, promising for advanced diagnostics.