Intolerance of uncertainty enhances adolescent fear generalization in both perceptual-based and category-based tasks:...

Fear generalization undergoes marked changes during adolescence, which may relate to the high prevalence of anxiety disorders. While intolerance of uncertainty (IU) is a key factor that amplifies fear generalization, its impact and neural basis in adolescence remain unclear. Here, we investigated the effects of IU on perceptual-based (n = 72) and category-based (n = 68) fear generalization in adolescents aged 12-15 years. Specifically, Experiment 1 utilized two different size rings as conditioned stimuli, with middle-sized rings serving as the generalized stimuli; Experiment 2 employed pictures of sparrows and refrigerators as conditioned stimuli, with other animals categorically related to the sparrow as generalized stimuli. We collected self-reported threat expectancy, response times, and fear ratings in both experiments, and conducted functional near-infrared spectroscopy in Experiment 2. Results showed that high IU adolescents had higher threat expectancy in both experiments compare to low IU. Moreover, in category-based generalization, high IU adolescents had higher fear ratings, shorter response times and reduced engagement of the left dorsolateral prefrontal cortex (DLPFC). Results indicated that IU may enhance fear generalization by deficient left DLPFC recruitment. Together the present findings point to a behavioral and neural mechanism that can render adolescents vulnerable for mental disorders.