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Updated: Jun 7, 2025

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Engineering Robust Silver-Decorated calcium peroxide Nano-Antibacterial Platforms for chemodynamic enhanced
Ge Bai1, Chunhua Niu2, Xuexue Liang1
1State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, PR China.
Abstract:
Calcium peroxide (CaO2) is commonly used as a hydrogen peroxide (H2O2) donor to eliminate bacterial infections. However, the rapid dissociation of CaO2 and the explosive release of H2O2 have limited the development of CaO2 in the antibacterial field. Therefore, a series of silver nanoparticles (AgNPs) functionalized bacteria-triggered smart hydrogels (CSA-H) that integrate sustained release of nanoparticles and localized chemodynamic sterilization were constructed. The pH-responsive hydrogel formed through the Schiff base reaction enables the responsive release of CaO2 nanoparticles while simultaneously regulating the concentration of H2O2 within the bacterial infection microenvironment. AgNPs are capable of reacting with H2O2 under mildly acidic conditions to produce hydroxyl radicals with enhanced antimicrobial activity. The antimicrobial results demonstrated that AgNPs functionalized silicon dioxide-coated calcium peroxide (CaO2@SiO2/AgNPs) nanoparticles exhibited enhanced bactericidal activity compared to AgNPs or CaO2 alone. Furthermore, CSA-H hydrogels exhibited significant antibacterial activity against S. aureus and E. coli under the dual effect of AgNPs and pH-driven Fenton-like reactions. This chemodynamic antibacterial platform is environmentally responsive and provides a promising strategy for creating multifunctional hydrogels loaded with nano-enzymes, thus advancing the development of AgNPs in chemodynamic-antibacterial related applications.
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