Soil microbiological assessment on diversified annual cropping systems in China

Recent studies have demonstrated that monocropping of flue-cured tobacco can lead to various issues, including nutrient deficiencies, accumulation of allelopathic substances, and disturbance in soil microbial flora. While diversification in cropping systems has proven effective in alleviating monocropping barriers, however, further exploration is needed to understand the potential microbial mechanisms involved in this process. In our study, we set five cropping systems (RR: rice monocropping; TR: tobacco-rice rotation over 20 years; TRA: tobacco-rice-astragalus rotation; TRW: tobacco-rice-wheat rotation; TRO: tobacco-rice-oilseed rape rotation) to explore the impact on crop yield and quality, soil chemical properties, and microbial diversity. The results showed that the yield and gross margin were significantly decreased. Following diversification in cropping systems, particularly after implementing the TRA treatment, the yield and gross margin increased by 27.35% and 38.67%, respectively, compared to the TR treatment. Additionally, the presence of tobacco in the soil resulted in acidification, reduced soil fertility, and suppression of soil microorganism diversity and metabolite abundance. With diversification in cropping systems, there was an increase in soil pH, carbon and nitrogen cycle enzyme activities, and the relative abundance of beneficial microorganisms (acidobacteria, nitrospirillum, and ascomycota) and soil metabolites. Diversification in cropping systems has the potential to increase crop biomass, soil fertility, and soil microbial environment. Our results suggest a scientific foundation for implementing effective nutrient management practices and rational crop rotation systems.