Novel analytical approach for baclofen quantification in rodent plasma

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Baclofen is a muscle relaxant with critical applications in neurological disorders.
• Accurate quantification of Baclofen in biological matrices is essential for pharmacokinetic and bioavailability assessments.

Purpose of the Study:

• To develop and validate a simple, accurate, and robust reverse-phase high-performance liquid chromatography (RP-HPLC) method for Baclofen quantification in rat plasma.
• To establish a reliable analytical method suitable for routine laboratory use and pharmacokinetic studies.

Main Methods:

• Method development and validation using RP-HPLC.
• Quantification of Baclofen in rat plasma over a concentration range of 10-50 μg/mL.
• Evaluation of linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness according to ICH guidelines.

Main Results:

• The developed RP-HPLC method demonstrated high linearity with an R-squared value of 0.9936.
• The method achieved a low limit of detection (LOD) of 0.076197 μg/mL and a limit of quantification (LOQ) of 0.23090 μg/mL.
• The method proved to be robust, ensuring reproducibility across different conditions and analysts.

Conclusions:

• The validated RP-HPLC method offers an efficient and reliable approach for quantifying Baclofen in rat plasma.
• This method is well-suited for supporting pharmacokinetic and bioavailability studies, with optimized parameters for routine laboratory application.