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Morroniside Attenuates Rheumatoid Arthritis by Inhibiting Rheumatoid Synoviocytes Invasion through the NF-κB/MMPs

Yan Wang1,2, Ruili Yin1,2, Xin Li1,2

  • 1Center for Endocrine Metabolism and Immune Diseases, Beijing Luhe Hospital, Capital Medical University, Tongzhou District, Beijing, China.

Annals of Clinical and Laboratory Science
|November 13, 2024
PubMed
Abstract

Insights

Morroniside (MOR) effectively inhibits rheumatoid arthritis (RA) joint destruction by preventing fibroblast-like synoviocyte (FLS) invasion. It suppresses the NF-κB/MMPs pathway, offering a potential therapeutic strategy for RA.

Area of Science:

  • Pharmacology
  • Immunology
  • Rheumatology

Background:

  • Morroniside (MOR) shows anti-inflammatory effects in cardiovascular and cerebrovascular diseases.
  • The mechanism of MOR in rheumatoid arthritis (RA) and its effect on joint destruction are not well understood.

Purpose of the Study:

  • To investigate the therapeutic potential of morroniside in rheumatoid arthritis (RA).
  • To explore the anti-invasive mechanism of morroniside on joint destruction in RA.

Main Methods:

  • In vitro studies used primary rat articular fibroblast-like synoviocytes (FLSs) to assess migration and matrix metalloproteinase (MMP) expression (MMP2, MMP9).
  • Western blot and immunofluorescence were employed to analyze the NF-κB signaling pathway components.
  • In vivo studies utilized a collagen-induced arthritis (CIA) rat model to evaluate joint histopathology and MMP expression.

Main Results:

  • Morroniside reduced TNFα-stimulated FLS migration and attenuated joint invasion and destruction in CIA rats.
  • MOR inhibited the overexpression of MMP2 and MMP9 in both stimulated FLSs and CIA rat joints.
  • Mechanistically, morroniside suppressed IKKα/β activation, leading to increased levels of IκBα and inhibition of NF-κB.

Conclusions:

  • Morroniside prevents joint destruction in RA by inhibiting FLS invasion.
  • The anti-arthritic effect of MOR is mediated through the suppression of the NF-κB/MMPs pathway.
Keywords:
invasionmorronisidenuclear factor-Brheumatoid arthritissynoviocyte

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