Related Experiment Video

Updated: Jul 17, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

13.0K

[Etiological characteristics and whole genome sequence analysis of Clostridium perfringens causing a food poisoning

Y Yi1, K Guo1, H Xiao1

  • 1Qingdao Center for Disease Control and Prevention of Bacterial Disease Laboratory/Qingdao Institute of Prevention Medicine, ShandongQingdao266033, China.

Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine]
|November 13, 2024
PubMed

Insights

This study identified F-type Clostridium perfringens as the cause of a food poisoning outbreak linked to roujiamo sandwiches. Whole genome sequencing revealed the pathogen

Area of Science:

  • Foodborne illness microbiology
  • Genomic epidemiology
  • Bacterial pathogenesis

Context:

  • Investigating a food poisoning outbreak linked to 'roujiamo' sandwiches.
  • Identifying the etiological agent responsible for human illness.

Purpose:

  • To determine the characteristics of Clostridium perfringens (CP) involved in a food poisoning outbreak.
  • To perform whole genome sequencing of the causative CP strain.
  • To analyze virulence factors, drug resistance, and biological traits of the pathogen.

Summary:

  • Multiplex real-time PCR and nucleic acid tests identified CP.
  • Molecular typing using pulsed-field gel electrophoresis (PFGE) and PLC gene sequencing provided phylogenetic insights.
  • Whole genome sequencing of strain QD2022FB4-CP4 revealed active metabolism, multiple virulence genes, and multidrug resistance.

Impact:

  • Identified F-type Clostridium perfringens as the cause of the outbreak.
  • Characterized the pathogen's genomic features, including virulence and resistance.
  • Provides valuable data for understanding and preventing future foodborne CP outbreaks.

More Related Videos

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples08:00

Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Published on: October 18, 2019

20.0K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.1K

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Bacterial Toxins01:12

Bacterial Toxins

Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
Cholera01:25

Cholera

Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...