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Related Experiment Videos

Glucose-coupled sodium absorption in the developing rat colon.

G D Potter, S M Burlingame

    The American Journal of Physiology
    |February 1, 1986
    PubMed
    Summary

    The fetal rat colon absorbs sodium dependent on glucose at birth, a function lost by 6-8 days postpartum. This study investigates the developmental changes in colon transport mechanisms.

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    Area of Science:

    • Physiology
    • Developmental Biology
    • Gastroenterology

    Background:

    • The developing mammalian colon has absorptive capabilities at birth.
    • The timing of the loss of glucose transport capacity and its effect on sodium absorption in the rat colon remain unstudied.

    Purpose of the Study:

    • To investigate the developmental changes in sodium (Na) transport and transepithelial potential difference (PD) in the fetal and neonatal rat colon.
    • To determine the effect of glucose on Na absorption in the developing rat colon.

    Main Methods:

    • In vitro perfusion of colon segments from fetal (20-day-old) and neonatal (6-8 days old) rats.
    • Measurement of Na transport and PD using 22Na and perfusates containing glucose or mannitol.
    • Assessment of the effect of phlorizin and amiloride on Na transport and PD.

    Main Results:

    • Fetal rat colons (day 20) exhibited glucose-dependent Na flux and a significant increase in PD upon glucose addition, inhibited by phlorizin.
    • The glucose-stimulated Na transport and PD response were lost in rat colons by 6-8 days of age, despite persistent villus epithelium.
    • Amiloride did not affect PD or Na transport at either developmental stage.

    Conclusions:

    • The fetal rat colon possesses glucose-dependent Na transport mechanisms at birth.
    • This glucose-dependent Na transport capacity is transient and is lost shortly after birth (by 6-8 days of age).
    • The findings highlight a critical developmental window for nutrient-coupled ion transport in the mammalian colon.

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