Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

6
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
6
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

3
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
3
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diffractive axicons for quasi-achromatic, propagation-invariant tubular beams.

Optics express·2026
Same author

The mitochondria-targeted peptide HDAP2 reduces mitochondrial loss and retinal ganglion cell degeneration after optic nerve injury.

Neuroscience·2026
Same author

Mitochondrial-Targeted HDAP2 Preserves Retinal Ganglion Cells and Increases Pressure Tolerance in DBA/2J Mice.

Investigative ophthalmology & visual science·2026
Same author

Influence of object motion on roughness measurements using spectral speckle correlation.

Optics express·2025
Same author

Mitochondrial-Targeted HDAP2 Preserves Retinal Ganglion Cells and Increases Pressure Tolerance in DBA/2J Mice.

bioRxiv : the preprint server for biology·2025
Same author

Targeting High-Density Aromatic Peptides to Cardiolipin Optimizes the Mitochondrial Membrane Potential and Inhibits Oxidative Stress.

FASEB bioAdvances·2025
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.2K

Two-wavelength holographic micro-endoscopy.

Alexander Gröger, Robert Kuschmierz, Alexander Birk

    Optics Express
    |November 14, 2024
    PubMed
    Summary
    This summary is machine-generated.

    We developed a micro-endoscope for precise surface measurements using two-wavelength holography. A deep learning method significantly improved accuracy by reducing measurement errors in noisy phase maps.

    More Related Videos

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    2.5K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.2K

    Related Experiment Videos

    Last Updated: Jun 7, 2025

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.2K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    2.5K
    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
    12:54

    Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

    Published on: October 2, 2021

    3.2K

    Area of Science:

    • Optical Engineering
    • Metrology
    • Biomedical Imaging

    Background:

    • Accurate micro-scale surface topography measurement is crucial for various scientific and industrial applications.
    • Existing micro-endoscopic techniques face limitations in resolution and accuracy due to factors like fiber optic properties and noise.

    Purpose of the Study:

    • To present a novel micro-endoscopic topography measurement method using two-wavelength holography.
    • To assess the impact of fiber optic characteristics and introduce virtual surface roughness as a resolution limiter.
    • To develop and evaluate a compact micro-endoscope system with enhanced measurement accuracy.

    Main Methods:

    • Evaluation of inter-core dispersion and cross-talk in commercial imaging fiber bundles (CFBs).
    • Design and fabrication of a micro-endoscope (<450 µm diameter) using 3D-printed micro-optics.
    • Implementation of two-wavelength holography for topography measurement.
    • Application of a simple phase unwrapping approach and a deep learning method for error reduction.

    Main Results:

    • Characterization of CFB properties and the concept of virtual surface roughness.
    • Achieved a relative measurement error of 7.5% with a basic phase unwrapping method on a pyramid test object.
    • Demonstrated significant error reduction using a deep learning approach tailored for noisy phase maps.
    • The standard deviation of measurement error was reduced by 4.2 times with the deep learning method.

    Conclusions:

    • The developed micro-endoscope system enables accurate topography measurements at the micro-scale.
    • Two-wavelength holography combined with advanced processing (deep learning) offers a powerful solution for high-resolution metrology.
    • The deep learning approach effectively mitigates noise and improves the reliability of micro-endoscopic measurements.