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Oligomeric proanthocyanidin ameliorates sepsis-associated renal tubular injury: involvement of oxidative stress, inflammation, PI3K/AKT and NFκB signaling pathways

  • 0Department of Anesthesiology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210023, China.
The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology +

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November 14, 2024

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November 14, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Oligomeric proanthocyanidin (OPC) protects against sepsis-induced kidney injury by reducing oxidative stress and inflammation. This plant compound activates beneficial signaling pathways, offering a potential treatment for sepsis-related renal damage.

Area Of Science

  • Nephrology
  • Pharmacology
  • Toxicology

Background

  • Sepsis can lead to severe organ damage, particularly acute kidney injury.
  • Early intervention is crucial for improving outcomes in sepsis patients.
  • Oligomeric proanthocyanidin (OPC) shows promise for renal protection.

Purpose Of The Study

  • To investigate the renoprotective effects of OPC in a mouse model of sepsis-induced acute kidney injury.
  • To elucidate the underlying mechanisms of OPC's action against kidney damage.

Main Methods

  • Sepsis-related acute kidney injury was induced in C57/B6 mice using lipopolysaccharide (LPS).
  • Renal function, pathology, oxidative stress markers, and inflammatory cytokines were assessed.
  • RNA sequencing, MTT assays, Hoechst/propidium iodide staining, and Western blot were employed to analyze cellular and molecular changes.

Main Results

  • OPC treatment improved renal function and attenuated pathological damage in LPS-induced kidney injury.
  • OPC restored antioxidant capacity (glutathione, SOD, catalase) and reduced lipid peroxidation (MDA).
  • OPC suppressed pro-inflammatory cytokines, enhanced anti-inflammatory cytokines, and modulated PI3K/AKT and NFκB signaling pathways in renal cells.

Conclusions

  • OPC demonstrates significant renoprotective effects against LPS-induced acute kidney injury.
  • Mechanisms involve counteracting oxidative stress, inhibiting inflammation, and regulating PI3K/AKT and NFκB pathways.
  • OPC represents a potential therapeutic strategy for mitigating renal injury in sepsis patients.

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