Enrichment of acid-tolerant sulfide-producing microbes from an acidic pit lake
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View abstract on PubMed
Summary
This summary is machine-generated.Bioremediation of acidic pit lakes using sulfate-reducing bacteria (SRB) was explored. Adding glycerol and elemental sulfur significantly boosted sulfide production, enriching acid-tolerant SRB like Desulfosporosinus acididurans.
Area Of Science
- Environmental Science
- Microbiology
- Bioremediation
Background
- Acidic pit lakes contain high metal(loid) concentrations and extreme acidity.
- Bioremediation strategies are needed to mitigate these environmental concerns.
- Biosulfidogenesis is a key strategy for metal(loid) immobilization via sulfide mineral formation.
Purpose Of The Study
- To investigate bioremediation of the meromictic Cueva de la Mora pit lake.
- To stimulate sulfate reduction and sulfide production using amendments.
- To identify and enrich acid-tolerant sulfide-producing microbes.
Main Methods
- Testing amendments (glycerol, elemental sulfur, glycerol + elemental sulfur) to stimulate microbial sulfate reduction.
- Monitoring sulfide production.
- Analyzing microbial communities using omics-based approaches.
Main Results
- Glycerol addition rapidly stimulated dissimilatory sulfate reduction, indicating electron donor limitation.
- Combined glycerol and elemental sulfur yielded the highest sulfide production.
- Desulfosporosinus acididurans, an acid-tolerant SRB, was significantly enriched, dominating microbial communities.
Conclusions
- Electron donor availability limits biosulfidogenesis in acidic pit lakes.
- Combined electron donor and acceptor amendments enhance sulfide production.
- Desulfosporosinus acididurans is a promising candidate for bioremediation of acidic environments.
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