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Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes
Published on: August 24, 2018
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Exploring diabesity pathophysiology through proteomic analysis using Caenorhabditis elegans
Malaimegu Subhadra1, Dilawar Ahmad Mir1, Koley Ankita1
1Department of Biotechnology, Alagappa University, Karaikudi, Tamil Nadu, India.
Frontiers in Endocrinology
|November 14, 2024
Summary
Diabesity in C. elegans models shows high glucose and cholesterol disrupt lipid and glucose homeostasis, shortening lifespan and impairing motor function. This study reveals molecular and structural damage linked to diabesity.
Area of Science:
- Biomedical research
- Metabolic disorders
- Model organism studies
Background:
- Diabesity, or obesity-driven Type 2 diabetes mellitus (T2DM), results from complex genetic and environmental factors affecting metabolic homeostasis.
- Maintaining stable lipid and glucose levels is crucial for preventing metabolic dysfunction.
Purpose of the Study:
- To investigate the mechanistic underpinnings of diabesity using a Caenorhabditis elegans model.
- To assess the impact of diabesity on physiological functions, cellular integrity, and molecular pathways.
Main Methods:
- C. elegans were exposed to high glucose conditions to mimic diabetic hyperglycemia.
- Assays included triglyceride measurement, lifespan analysis, pharyngeal pumping, oxidative stress assessment, behavioral tracking, and dopamine signaling analysis.
- Proteomic analysis, microscopy, and immunofluorescence staining were used to evaluate protein expression, pathway dysregulation, and structural integrity.
Main Results:
- High glucose and cholesterol diets increased triglyceride levels, reduced lifespan, and decreased pharyngeal pumping in C. elegans.
- Diabesity induced oxidative stress (ROS), altered lipid/protein profiles, impaired dopamine signaling, and reduced motility.
- Proteomic analysis identified dysregulated pathways including insulin signaling and Alzheimer's disease pathways.
- Decreased collagen production led to anatomical disruptions.
Conclusions:
- Diabesity significantly impacts cellular components and structural integrity in C. elegans.
- The study provides insights into the molecular and physiological mechanisms underlying diabesity-associated metabolic disorders.
