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Updated: Oct 3, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Determination of calmodulin by competitive binding assay
Abstract:
Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.
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