Effect of caffeine on motility of buffalo spermatozoa in vitro

Addition of an optimum level of caffeine (7 mM) during preservation (5 degrees C) of buffalo semen in EYC medium had a stimulatory effect on sperm motility. It increased the preservation time of buffalo semen by delaying the loss of sperm motility. Immotile buffalo sperm due to cold shock could also be mobilised by addition of caffeine.