Field Trials of an Autonomous eDNA Sampler in Lotic Waters.

Area of Science:

• Environmental science
• Molecular biology
• Ecology

Background:

• Environmental DNA (eDNA) analysis is a powerful tool for biodiversity assessment.
• Current eDNA sample collection methods lack standardization and require significant field effort.
• High-frequency sampling is needed to capture temporal eDNA dynamics.

Purpose of the Study:

• To evaluate the utility of autonomous eDNA samplers for freshwater biosurveillance.
• To assess the insights gained from high-frequency eDNA autosampling.
• To benchmark autonomous sampler performance against manual sampling methods.

Main Methods:

• Deployed a prototype autonomous eDNA sampler in various riverine habitats.
• Conducted short-term deployments for high-frequency water sampling.
• Compared autosamples with manually collected samples for eDNA detection and quantity.
• Analyzed eDNA concentrations in relation to environmental covariates like discharge and turbidity.

Main Results:

• High-frequency autosampling revealed significant temporal variability in eDNA concentrations.
• Autosamples demonstrated similar or higher eDNA detection rates and quantities compared to manual samples.
• eDNA concentrations showed associations with environmental covariates such as discharge and turbidity.
• Minimal carryover contamination was observed in autosampler field controls.

Conclusions:

• Autonomous eDNA samplers can improve freshwater biosurveillance by overcoming logistical barriers.
• High-frequency sampling with autosamplers aids in standardizing collection methods.
• Autosamplers provide valuable insights into eDNA dynamics and environmental influences.
• This technology has the potential to enhance the efficiency and effectiveness of ecological monitoring.