Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The de Broglie Wavelength02:32

The de Broglie Wavelength

25.3K
In the macroscopic world, objects that are large enough to be seen by the naked eye follow the rules of classical physics. A billiard ball moving on a table will behave like a particle; it will continue traveling in a straight line unless it collides with another ball, or it is acted on by some other force, such as friction. The ball has a well-defined position and velocity or well-defined momentum, p = mv, which is defined by mass m and velocity v at any given moment. This is the typical...
25.3K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.6K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.4K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.4K
The Quantum-Mechanical Model of an Atom02:45

The Quantum-Mechanical Model of an Atom

42.0K
Shortly after de Broglie published his ideas that the electron in a hydrogen atom could be better thought of as being a circular standing wave instead of a particle moving in quantized circular orbits, Erwin Schrödinger extended de Broglie’s work by deriving what is now known as the Schrödinger equation. When Schrödinger applied his equation to hydrogen-like atoms, he was able to reproduce Bohr’s expression for the energy and, thus, the Rydberg formula governing hydrogen spectra.
42.0K
The Wave Nature of Light02:12

The Wave Nature of Light

48.4K
The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion. 
48.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tautomerism unveils a self-inhibition mechanism of crystallization.

Nature communications·2023
Same author

Probing atom dynamics of excited Co-Mo-S nanocrystals in 3D.

Nature communications·2021
Same author

Step edge structures on the anatase TiO<sub>2</sub> (001) surface studied by atomic-resolution TEM and STM.

Faraday discussions·2018
Same author

Comment on, "On the influence of the electron dose-rate on the HRTEM image contrast", by Juri Barthel, Markus Lentzen, Andreas Thust, ULTRAM12246 (2016), http://dx.doi.org/10.1016/j.ultramic.2016.11.016.

Ultramicroscopy·2017
Same author

In situ studies of NO reduction by H<sub>2</sub> over Pt using surface X-ray diffraction and transmission electron microscopy.

Physical chemistry chemical physics : PCCP·2017
Same author

Detecting structural variances of Co<sub>3</sub>O<sub>4</sub> catalysts by controlling beam-induced sample alterations in the vacuum of a transmission electron microscope.

Advanced structural and chemical imaging·2016

Related Experiment Video

Updated: Jun 7, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.2K

Single Electron Self-coherence and Its Wave/Particle Duality in the Electron Microscope.

C Kisielowski1,2, P Specht1,3, J R Jinschek4,5

  • 1Electron Scattering Solutions, 337 Moraga Ave, Piedmont, CA 94611, USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|November 14, 2024
PubMed
Summary

Electron microscopy reveals that coherent crystal illumination and phase contrast are lost when self-coherence length falls below the unit cell size. This occurs at energy losses over 200 eV, impacting high-resolution imaging.

Keywords:
Heisenbergs Uncertainty Principlecoherenceinelastic scatteringtime-dependent Schrödinger equationwave/particle duality

More Related Videos

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

9.7K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K

Related Experiment Videos

Last Updated: Jun 7, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.2K
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

9.7K
Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

7.7K

Area of Science:

  • Physics
  • Materials Science
  • Electron Microscopy

Background:

  • Electron microscopy relies on detecting single electrons for image formation.
  • Coherent-inelastic interactions involve time-dependent partial waves and phase decoherence.
  • Heisenberg's uncertainty principle relates energy uncertainty to interaction time.

Purpose of the Study:

  • To investigate the relationship between self-coherence length and image quality in electron microscopy.
  • To determine the energy loss threshold at which coherent illumination and phase contrast are compromised.
  • To explore the generalizability of coherent-inelastic interaction concepts.

Main Methods:

  • Utilizing the Goos-Hänchen shift to measure self-coherence length.
  • Employing chromatic-aberration corrected electron microscopy.
  • Analyzing boron nitride (BN) samples.

Main Results:

  • Self-coherence length was experimentally measured.
  • Coherent crystal illumination and phase contrast were lost when self-coherence length was smaller than the unit cell size.
  • This loss occurred at energy losses greater than 200 eV.

Conclusions:

  • The study establishes a critical energy loss threshold impacting coherent imaging in electron microscopy.
  • The findings suggest that the concept of coherent-inelastic matter wave interactions may be broadly applicable.
  • Understanding self-coherence length is crucial for high-resolution phase-contrast imaging.