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An innovative fluorescent probe for monitoring of ONOO- in multiple liver-injury models

  • 0School of Chemistry and Chemical Engineering, Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources, Collaborative Innovation Center of Ecological Civilization, Hainan University, Haikou, 570228, PR China.
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November 14, 2024

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November 14, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Researchers developed NAP-ONOO, a novel fluorescent probe, to detect peroxynitrite (ONOO-) levels during liver injury. This tool aids in identifying liver damage and screening drugs in various models.

Area Of Science

  • Biochemistry
  • Chemical Biology
  • Toxicology

Background

  • The liver is vital for metabolism and detoxification; chronic liver disease leads to severe health issues.
  • Abnormal peroxynitrite (ONOO-) concentrations are linked to liver disease onset and progression.
  • Detecting reactive ONOO- in physiological settings is challenging.

Purpose Of The Study

  • To develop an innovative fluorescent probe for real-time monitoring of ONOO-.
  • To assess the probe's efficacy in various liver injury models.

Main Methods

  • Synthesis of NAP-ONOO, a 1,8-naphthalimide-derived fluorescent probe.
  • Application of NAP-ONOO in cellular systems, zebrafish, and mice models.
  • Evaluation of ONOO- up-regulation in models of inflammatory, alcohol-induced, and drug-induced liver injury.

Main Results

  • NAP-ONOO demonstrated high biocompatibility and effective dynamic monitoring of ONOO- fluctuations.
  • The probe successfully observed ONOO- up-regulation in diverse liver injury models.
  • NAP-ONOO facilitated the identification of ONOO- as a liver injury biomarker.

Conclusions

  • NAP-ONOO is a valuable tool for detecting ONOO- and identifying liver injuries.
  • The probe shows potential for drug screening and cellular imaging in liver research.
  • This work opens new avenues for understanding and managing liver disease.

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