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Updated: Jun 7, 2025
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs)
Silvia Selvitella1, Marta Pérez Franco1, Begoña Aran1
1Barcelona Stem Cell Bank, Spain; Regenerative Medicine Program, Institut d'Investigació Biomèdica, IDIBELL, Spain; P-CMR[C]-Program of Translation of Regenerative Medicine in Catalonia, Spain.
Abstract:
mRNA reprogramming is a technology for generating iPSCs with high efficiency and safety. However, it is not suitable for reprogramming non-adherent cells, the primary cell type in blood. An alternative is to obtain adherent cells from blood for this technology. To validate this approach, we generated a human iPSC line from blood outgrowth endothelial cells (BOECs) using mRNA-based reprogramming. The resulting line, CTRL EiPS J9 mR6F-8, meets iPSC criteria, including an undifferentiated state, pluripotency, genome integrity. This line is available upon request.
