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Aurora kinase as a putative target to tick control.

Bruno Moraes1,2, Helga Gomes3, Luiz Saramago2

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Summary

Aurora kinases are crucial for cell division. In ticks, specific sequences in Rm-AURKA offer potential for targeted inhibition, impacting parasite control.

Keywords:
Aurora-kinaseBME26CCT137690Rhipicephalus microplusembryonic celltick

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Area of Science:

  • Molecular Biology
  • Parasitology
  • Biochemistry

Background:

  • Aurora kinases (AURK) are serine-threonine proteins vital for cell cycle regulation, including mitosis.
  • Mammals possess three AURK isoforms (A, B, C) with distinct cell cycle roles.
  • Two AURK sequences (Rm-AURKA, Rm-AURKB) were identified in the cattle tick *Rhipicephalus microplus*, with highest expression in ovaries.

Purpose of the Study:

  • To investigate the role and potential inhibition of Aurora kinases in the cattle tick *Rhipicephalus microplus*.
  • To identify species-specific differences in AURK sequences for targeted therapeutic development.
  • To explore the efficacy of a pan-AURK inhibitor against tick cells.

Main Methods:

  • Transcriptome analysis to identify AURK sequences in *R. microplus*.
  • Bioinformatic analysis of deduced amino acid sequences, comparing tick and vertebrate AURKs.
  • Cell viability assays using a pan-AURK inhibitor (CCT137690) on tick cell lines.
  • In silico molecular docking to assess inhibitor-target interactions.

Main Results:

  • Non-conserved threonine residues, essential for vertebrate AURK function, were identified in *R. microplus* sequences.
  • The pan-AURK inhibitor CCT137690 reduced viability in the BME26 tick cell line.
  • In silico analysis revealed exclusive interaction sites between CCT137690 and Rm-AURKA.

Conclusions:

  • The identified non-conserved residues and exclusive interaction sites in Rm-AURKA present opportunities for developing species-specific inhibitors.
  • Targeting tick Aurora kinases could lead to novel strategies for controlling ectoparasites like *Rhipicephalus microplus*.
  • Further research into these exclusive regions can facilitate the design of targeted therapies against ticks.