Mouse neuroblastoma cloning efficiency modulation by quiescent 3T3 cells in culture

In the mixed culture system nontransformed 3T3 fibroblasts cells inhibited the plating efficiency of neuroblastoma (N2a) cells, whereas heat or methanol killed fibroblasts did not. In contrast, chloroform/methanol extracted 3T3 cells inhibited the plating efficiency of neuroblastoma cells to the same extent as had been the case with living 3T3 cells. In the present study, we discuss the possibility that nontransformed cells surface components can modify the proliferation of neuroblastoma cells.