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Updated: Jun 7, 2025

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Framework Nucleic Acid-Based Selective Cell Catcher for Endogenous Stem Cell Recruitment.

Xingyu Chen1,2, Ziang Xu1,2, Yang Gao1,2

  • 1State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, China.

Advanced Materials (Deerfield Beach, Fla.)
|November 15, 2024
PubMed
Summary
This summary is machine-generated.

This study uses programmable tetrahedral framework nucleic acids (tFNAs) to improve stem cell attraction for tissue regeneration. The engineered tFNA hydrogel system effectively captures mesenchymal stem cells (MSCs) and accelerates bone healing.

Keywords:
aptamer 19Sbone repairhyaluronic acid hydrogelstem cell therapytetrahedral framework nucleic acid

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Cell-surface engineering aims to enhance stem cell recruitment for tissue regeneration.
  • Challenges include ligand internalization and dynamic cell membranes affecting interactions.
  • Existing methods require precise control over ligand-receptor dynamics.

Purpose of the Study:

  • To utilize programmable tetrahedral framework nucleic acids (tFNAs) for tunable ligand-receptor interactions.
  • To improve stem cell recruitment efficiency using engineered tFNAs.
  • To develop an efficient stem cell catcher system for bone regeneration.

Main Methods:

  • Experimental screening and dissipative particle dynamics for theoretical analysis.
  • Modification of tFNA ligand flexibility and topology.
  • Optimization of mesenchymal stem cell (MSC)-binding aptamer 19S (Apt19S) distribution on tFNA.
  • Chemical linkage of Apt19S-tFNA to hyaluronic acid hydrogel.

Main Results:

  • tFNA ligand properties influence cellular internalization and membrane binding.
  • Optimized Apt19S distribution on tFNA enhances MSC capture efficiency.
  • The Apt19S-tFNA hydrogel system successfully captures MSCs in vitro.
  • In vivo studies show accelerated bone regeneration in cranial and maxillary defects.

Conclusions:

  • Programmable tFNAs offer precise control over ligand-receptor interactions for stem cell recruitment.
  • The developed hydrogel system acts as an efficient 'stem cell catcher'.
  • This approach shows significant potential for enhancing endogenous stem cell-based tissue regeneration, particularly in bone healing.