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Methods for isolating Campylobacter jejuni from low-turbidity water.

M J Blaser, H J Cody

Applied and Environmental Microbiology

|February 1, 1986

Summary

This study optimized membrane filtration for recovering Campylobacter jejuni in low-turbidity water. The best method involved plating filters facedown on selective agar for accurate detection.

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Area of Science:

Environmental microbiology
Water quality analysis
Bacteriology

Background:

Campylobacter jejuni is a significant cause of bacterial gastroenteritis.
Accurate detection of C. jejuni in water is crucial for public health.
Existing methods for C. jejuni recovery from environmental water can be challenging.

Purpose of the Study:

To develop and evaluate membrane filtration techniques for quantitative recovery of C. jejuni.
To identify the most sensitive and reliable method for detecting C. jejuni in low-turbidity waters.
To provide a practical method for routine water monitoring and outbreak investigations.

Main Methods:

Development and comparison of various membrane filtration procedures.
Use of 0.45-micron pore size filters for water sample processing.
Incubation of filters facedown on Campylobacter-selective agar, followed by streaking for isolation.
Evaluation of prefiltration steps and filter orientation (face-up vs. facedown).

Main Results:

The optimal method involved passaging water through a 0.45-micron filter, plating it facedown on selective agar, and reincubating after streaking.
This technique consistently recovered 30 C. jejuni Colony Forming Units (CFU) per 250 ml in seeded natural waters.
Prefiltration using 5.0- and 0.6-micron membranes did not significantly alter recovery rates.
Plating filters face-up or pre-enriching filters in a medium yielded lower sensitivity.

Conclusions:

A highly sensitive membrane filtration method was established for C. jejuni recovery from low-turbidity environmental waters.
The optimized technique is suitable for routine monitoring of finished water and investigating suspected waterborne outbreaks.
This method enhances the ability to detect C. jejuni, contributing to improved water safety and public health surveillance.

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