Related Experiment Video
Updated: Jul 18, 2026

06:59
Intrathecal Application of a Fluorescent Dye for the Identification of Cerebrospinal Fluid Leaks in Cochlear Malformation
Published on: February 29, 2020
8.1K
Detection of Oronasal Fistula Using a Fluorescent Water Passing Test.
Masao Ogawa1,2, Seiya Yamaki1, Masamichi Yamashita2
1Amica Pet Clinic, Ube, Yamaguchi, Japan.
Journal of Veterinary Dentistry
|November 15, 2024
Summary
A new fluorescent water-passing test effectively detects small oronasal fistulas (ONFs) in dogs. This method uses fluorescein sodium to enhance visualization of fluid discharge, aiding in definitive diagnosis.
Area of Science:
- Veterinary Medicine
- Diagnostic Imaging
- Ophthalmology
Background:
- Oronasal fistulas (ONFs) connect the oral and nasal cavities, posing diagnostic challenges, especially when small.
- Visual detection of small ONFs is often difficult, necessitating improved diagnostic methods.
Purpose of the Study:
- To evaluate the efficacy of fluorescein sodium in detecting small oronasal fistulas (ONFs) in dogs.
- To assess the utility of a fluorescent "water-passing test" for ONF diagnosis.
Main Methods:
- The study involved 25 dogs with confirmed periodontal pockets.
- A fluorescent water-passing test was performed by injecting fluorescein sodium into periodontal pockets and observing for discharge from the external naris.
- Fluid discharge was detected using an examination light.
Main Results:
- Of 13 dogs with suspected ONFs, 12 tested positive with the fluorescent water-passing test.
- Among 12 asymptomatic dogs, 3 tested positive, indicating potential subclinical ONFs.
- The test enhanced visibility and facilitated detection of small fluid volumes.
Conclusions:
- The fluorescent water-passing test is a useful and effective method for detecting small oronasal fistulas (ONFs) in dogs.
- This diagnostic technique improves the ability to identify even subtle ONFs, aiding in timely and accurate diagnosis.

