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Techniques for Imaging Ca2+ Signaling in Human Sperm
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Sperm quality analyzer: A portable LED array microscope with dark-field imaging.

Meng Shao1, Changxu Li1, Xiaohao Ma1

  • 1School of Biomedical Engineering Anhui Medical University Hefei China.

Bioengineering & Translational Medicine
|November 15, 2024
PubMed
Summary
This summary is machine-generated.

A portable, low-cost microscope using encoded LED illumination and dark-field imaging enables accurate sperm quality analysis. This system, with its LabCASA software, offers a cost-effective solution for point-of-care testing, enhancing infertility diagnostics.

Keywords:
LED array microscopeLabCASAdark‐field imagingsperm motility

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Area of Science:

  • Biomedical Engineering
  • Reproductive Biology
  • Microscopy

Background:

  • Sperm quality analysis is crucial for infertility diagnosis, typically using expensive and bulky computer-assisted sperm analysis (CASA) systems.
  • Existing CASA systems face challenges in cost and portability for point-of-care testing (POCT).

Purpose of the Study:

  • To develop a low-cost, portable microscope for sperm quality analysis suitable for POCT.
  • To create a CASA software compatible with the portable microscope for assessing sperm motility.

Main Methods:

  • Utilized encoded light-emitting diode (LED) array illumination for multicontrast imaging.
  • Developed a portable microscope (16.5 × 14.0 × 25.0 cm) with dark-field (DF) imaging.
  • Created LabCASA software for sperm motility analysis using DF imaging data.

Main Results:

  • The portable microscope with DF imaging provided high-contrast sperm images suitable for CASA.
  • LabCASA software demonstrated high reliability, with motility parameter differences <10% CV compared to TrackMate.
  • The system showed robustness in tracking sperm motion and confirmed motility parameter variations with temperature.

Conclusions:

  • The developed portable microscope system with DF imaging and LabCASA software is a cost-effective solution for sperm quality analysis.
  • This system is well-suited for portable POCT of sperm quality, especially in resource-constrained settings.
  • The dark-field imaging approach enhances robustness and accuracy for sperm motility assessment.