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Updated: Jun 7, 2025

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Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
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A red turn-on fluorescent probe reveals elevated H2O2 in cell anesthesia
Longjie Du1, Yu Ning1, Wentao Li1
1School of Pharmacy, School of Basic Medical Sciences, Shandong Second Medical University, Weifang 261053, China. wfmcwjh@sdsmu.edu.cn.
Abstract:
Cognitive dysfunction has become an important central nervous system (CNS)-related adverse reaction of intravenous anesthetics. Therefore, to further understand the role of H2O2, a potential marker of cognitive dysfunction, we constructed a fluorescent probe (LJ-1), which could specifically enable real-time detection of H2O2 in cells during anesthesia.

