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Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
Published on: November 25, 2022
A distinct Borrelia clade within the relapsing fever group from cat-infesting Haemaphysalis semermis Neumann 1901
Abdul Rahman Kazim1, Chong Chin Heo1, Shezryna Shahrizal2
1Department of Medical Microbiology and Parasitology, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Sungai Buloh Campus, Sungai Buloh, 47000, Selangor, Malaysia.
Abstract:
An undescribed relapsing fever group Borrelia species was detected in a male Haemaphysalis semermis tick infesting a rural cat in an indigenous population in Pahang National Park, Peninsular Malaysia. The 16 S rRNA gene sequence revealed close similarity of this variant to several undescribed Borrelia species and Borrelia theileri, with genetic distances ranging from 0.58 to 0.72%. Furthermore, the flaB, gyrB, and the concatenated 16 S rRNA + flaB + gyrB sequence analyses demonstrated that this variant is distinctly separated from multiple undescribed Borrelia species, Borrelia miyamotoi, and B. theileri, with genetic distances ranging from 3.41 to 7.00%. This study not only reports the first Borrelia found in H. semermis but also suggests that it forms a distinct clade within the relapsing fever group in Peninsular Malaysia.
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