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Methodology for Biological Sample Collection, Processing, and Storage in the Newcastle 1000 Pregnancy Cohort:
Joshua J Fisher1, Tegan Grace1, Nathan A Castles1
1School of Medicine and Public Health, College and Health, Medicine and Wellbeing, The University of Newcastle, Callaghan, Australia.
JMIR Research Protocols
|November 15, 2024
Summary
The Newcastle 1000 Study (NEW1000) is creating a comprehensive biobank of biological samples from pregnant women and their partners. This resource will support research into the developmental origins of noncommunicable diseases across generations.
Area of Science:
- Developmental Origins of Health and Disease (DOHaD)
- Population-based pregnancy cohort studies
- Multigenerational health research
Background:
- Adverse events in the first 1000 days impact life course health.
- Mechanisms linking early life exposures to later health outcomes require further elucidation.
- The Newcastle 1000 Study (NEW1000) addresses this knowledge gap.
Purpose of the Study:
- To establish a comprehensive, prospective pregnancy cohort study (NEW1000).
- To investigate the developmental origins of noncommunicable diseases.
- To provide longitudinal, multisystem phenotyping with robust clinical data and biological samples.
Main Methods:
- Detailed description of sample collection, storage, and utility for the NEW1000 biobank.
- Focus on integrating samples into large-scale 'omics' platforms (genome, microbiome, epigenome, etc.).
- Collection of maternal, paternal, and fetal blood, microbiome, urine, saliva, hair, nails, placenta, colostrum, and breastmilk.
Main Results:
- Recruitment commenced March 2021; as of July 2024, 1040 women and 684 partners enrolled, 922 infants born.
- The NEW1000 biobank houses extensive sample collections, including plasma, buffy coat, serum, urine, microbiome swabs, saliva, breastmilk, placenta, hair, and nail samples.
- Detailed inventory of over 24,000 plasma aliquots and extensive microbiome and urine samples.
Conclusions:
- NEW1000 is generating a deeply phenotyped, multigenerational cohort.
- The comprehensive biobank supports diverse analyses across multiple 'omics' platforms.
- This resource is crucial for advancing understanding of developmental origins of noncommunicable diseases.
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