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Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
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Exploration of a miRNA-mRNA network shared between acute pancreatitis and Epstein-Barr virus infection by integrated

Xing Wei1, Zhen Weng2,3, Xia Xu4

  • 1Department of Infectious Disease, The Nantong First People's Hospital and The Affiliated Hospital 2 of Nantong University, Nantong, China.

Plos One
|November 15, 2024
PubMed

Insights

This study identified shared genes and microRNAs (miRNAs) between acute pancreatitis (AP) and Epstein-Barr virus (EBV) infection. A novel miRNA-mRNA network offers insights into molecular mechanisms and potential therapeutic targets for both conditions.

Area of Science:

  • Genomics
  • Molecular Biology
  • Virology

Background:

  • Acute pancreatitis (AP) is a significant cause of hospitalization with diverse etiologies, including Epstein-Barr virus (EBV) infection.
  • The shared molecular pathways and genetic underpinnings between AP and EBV infection are not well understood.
  • Identifying common molecular players could reveal novel therapeutic targets and diagnostic markers.

Purpose of the Study:

  • To identify common differentially expressed genes (DEGs) and microRNAs (DEMs) between AP and EBV infection.
  • To construct a miRNA-mRNA regulatory network and a competitive endogenous RNA (ceRNA) network.
  • To explore potential therapeutic interventions and immune cell infiltration patterns associated with shared molecular mechanisms.

Main Methods:

  • Downloaded and analyzed four Gene Expression Omnibus (GEO) datasets (GSE194331, GSE42455, GSE45918, GSE109220).
  • Performed differential expression analysis to identify DEGs and DEMs.
  • Constructed miRNA-mRNA and ceRNA networks, conducted enrichment analysis, drug prediction, and immune cell infiltration analysis.

Main Results:

  • Identified 111 shared genes and 8 shared miRNAs between AP and EBV infection.
  • Developed a miRNA-mRNA network with 5 miRNAs and 10 genes showing strong diagnostic potential.
  • Predicted Histone Deacetylase (HDAC) inhibitors as potential therapeutic agents.
  • Observed significant differences in activated B cell infiltration in AP and EBV-infected individuals compared to controls.

Conclusions:

  • This study elucidates, for the first time, a shared miRNA-mRNA network between AP and EBV infection.
  • The findings provide a deeper understanding of the molecular mechanisms underlying these conditions.
  • The identified network and HDAC inhibitors represent potential avenues for future therapeutic strategies and diagnostics.

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