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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Multicolor Gold Clusterzyme-Enabled Construction of Ratiometric Fluorescent Sensor Array for Visual Biosensing.

Xiaomeng Zhou1, Saijin Huang1, Wenfeng Guo2

  • 1State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

Analytical Chemistry
|November 15, 2024
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This study introduces a novel fluorescent sensor array using gold nanoclusters (AuNCs) for detecting multiple phosphates. This clusterzyme-based ratiometric sensor array enables visual, on-site disease diagnosis, demonstrated by identifying urinary tract infections.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Simultaneous detection of similar bioanalytes is challenging for research and diagnosis.
  • Existing biosensor arrays face issues with probe robustness, matrix interference, and on-site analysis.
  • Gold nanoclusters (AuNCs) possess intrinsic fluorescence and enzyme-mimic properties.

Purpose of the Study:

  • To develop an innovative ratiometric sensor array for enhanced fluorescent visual biosensing.
  • To address the challenge of simultaneous detection of multiple bioanalytes with similar structures.
  • To create a portable and visually interpretable biosensing platform for practical applications.

Main Methods:

  • Utilized gold nanoclusters (AuNCs) with distinct fluorescence colors (blue, green, red) and peroxidase-mimic activity.
  • Designed a ratiometric sensor array where phosphate presence alters AuNC fluorescence and enzymatic activity.
  • Integrated fluorescent clusterzymes into a hydrogel matrix for a portable sensor array.

Main Results:

  • The clusterzyme-based ratiometric sensor array effectively discriminated and detected eight types of phosphates.
  • Distinct optical responses were observed in a ratiometric manner due to phosphate-induced changes in AuNCs.
  • The portable hydrogel-integrated sensor array visually identified phosphates via fluorescence color changes.
  • Point-of-care diagnosis of urinary tract infections was achieved by analyzing urinary microbial ATP.

Conclusions:

  • The developed fluorescent clusterzyme-based ratiometric sensor array shows great potential for enhanced visual biosensing.
  • This platform offers a promising solution for discriminating and detecting multiple bioanalytes, overcoming limitations of current methods.
  • The strategy enables visual, on-site diagnosis, exemplified by the successful point-of-care detection of urinary tract infections.