Genetically modified organoids for tissue engineering and regenerative medicine
Qinmeng Zhang1, Jin He1, Danji Zhu1
1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310000, China.
Advances in Colloid and Interface Science
|November 15, 2024
Summary
Genetically modified organoids show promise for regenerative medicine by enabling gene delivery for tissue regeneration. Despite challenges in safety and bionics, they offer potential treatments for diseases like Fabry disease.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Tissue Engineering
Background:
- Genetically modified organoids are advanced 3D models for studying genetic diseases.
- They serve as effective vehicles for gene delivery to modulate gene expression.
- Preclinical studies highlight their role in regenerating various organs.
Purpose of the Study:
- To review recent advancements in genetically modified organoids.
- To detail gene transfection methods and functional applications.
- To discuss limitations and future directions in the field.
Main Methods:
- Review of current research on genetically modified organoids.
- Analysis of gene transfection techniques (viral and non-viral).
- Examination of organoid applications in disease treatment and organ regeneration.
Main Results:
- Genetically modified organoids facilitate gene expression modulation for tissue regeneration.
- Demonstrated efficacy in regenerating organs like the thymus, brain, and kidney.
- Potential therapeutic applications for genetic disorders including Fabry disease and Lynch syndrome.
Conclusions:
- Genetically modified organoids are a promising tool for regenerative medicine and disease treatment.
- Further research is needed to address safety, application risks, and bionic integration.
- Future development will focus on overcoming current limitations for broader clinical use.
