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Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

7.2K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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The Proteasome02:18

The Proteasome

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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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The Proteasome Structure01:17

The Proteasome Structure

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The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
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Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

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Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
12.0K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
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Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

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After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
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Related Experiment Video

Updated: Jun 7, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

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SnapShot: Targeted protein degradation.

Yu Ding1, Boxun Lu1

  • 1State Key Laboratory of Medical Neurobiology, Neurology Department at Huashan Hospital, School of Life Sciences, Fudan University, Shanghai 200433, China.

Cell
|November 15, 2024
PubMed
Summary

Targeted protein degradation uses cell machinery to remove specific proteins. New technologies advance drug discovery and studying intracellular, membrane, and extracellular proteins.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Targeted protein degradation harnesses the cell's natural systems to eliminate specific proteins.
  • This approach offers a novel strategy for modulating protein function and abundance.

Purpose of the Study:

  • To explore emerging technologies in targeted protein degradation.
  • To highlight applications in drug discovery and functional characterization of various protein types.

Main Methods:

  • Leveraging endogenous cellular degradation pathways.
  • Development and application of novel degradation-targeting technologies.

Main Results:

  • Demonstrated success in selectively degrading proteins of interest.

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Tuning Degradation to Achieve Specific and Efficient Protein Depletion
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Tuning Degradation to Achieve Specific and Efficient Protein Depletion

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Assaying Proteasomal Degradation in a Cell-free System in Plants
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Assaying Proteasomal Degradation in a Cell-free System in Plants

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Related Experiment Videos

Last Updated: Jun 7, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

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Tuning Degradation to Achieve Specific and Efficient Protein Depletion
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Tuning Degradation to Achieve Specific and Efficient Protein Depletion

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Assaying Proteasomal Degradation in a Cell-free System in Plants
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  • Opened new avenues for functional studies of intracellular, membrane, and extracellular proteins.

    • Conclusions:

    • Targeted protein degradation is a powerful tool with broad applications.
    • Emerging technologies are expanding the scope and efficacy of protein degradation strategies.