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Impact of cellulolytic nitrogen-fixing composite inoculants on humification pathways and nitrogen cycling in kitchen
Haimin Li1, Changqing Liu2, Ji-Qin Ni3
1College of Environment and Resources, College of Carbon Neutral Modern Technology, Fujian Normal University; Pollution Control and Resource Recycling Laboratory of Fujian Province, Fuzhou 350007, China; Fujian College and University Engineering Research Center for Municipal Solid Waste Resourceization and Management, Fuzhou 350007, Fujian, China.
Abstract:
Low humification and nitrogen loss pose substantial challenges to the resource utilization in kitchen waste composting. This study investigated the effects of brown-rot fungi (BRF), cellulolytic nitrogen fixing bacteria (CNFB), and their composite microbial inoculants (CMI) during composting. Results indicated that microbial inoculants extended the thermophilic phase and enhanced cellulose degradation. Compared with the control, the degree of polymerization (HA/FA) in BRF, CNFB, and CMI was 2.28, 1.85, and 2.68 times higher, respectively, while increasing total nitrogen by 11.15%, 15.50%, and 19.73%. BRF and CMI primarily enhanced the Maillard humification pathway, while CNFB promoted the polyphenol humification pathway. Additionally, BRF enhanced nitrification and reduced denitrification, whereas CNFB and CMI improved nitrification, nitrogen fixation, and ammonification while reducing denitrification. Overall, BRF primarily promoted humification, while CNFB excelled in nitrogen retention. The CMI achieved optimal humification and nitrogen retention, indicating a potential sustainable solution for kitchen waste composting.
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