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Comparative transcriptomic and metabolomic analysis of FTO knockout and wild-type porcine iliac artery endothelial cells

  • 0Beijing Laboratory Animal Research Center, Co., Ltd., Beijing 102609, China; Beijing Academy of Science and Technology, Beijing 100089, China.
Gene +

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November 15, 2024

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November 15, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

The fat mass and obesity associated (FTO) gene knockout reveals significant changes in cell gene expression and metabolism. This study uncovers FTO

Area Of Science

  • Molecular Biology
  • Genetics
  • Metabolomics

Background

  • The fat mass and obesity associated (FTO) gene is a key genetic factor in adiposity.
  • Emerging evidence links the FTO gene to various cancer types.
  • Understanding FTO's cellular functions is crucial for its role in disease.

Purpose Of The Study

  • To investigate the FTO gene's function and impact on cellular processes.
  • To analyze gene expression and metabolic changes in FTO-deficient cells.
  • To explore the molecular mechanisms underlying FTO's role in health and disease.

Main Methods

  • Established an FTO knockout (KO) cell line in porcine iliac artery endothelial cells (PIECs) using CRISPR/Cas9 technology.
  • Conducted comprehensive transcriptomic and metabolomic analyses to compare FTO KO and wild-type (WT) cells.
  • Performed enrichment analysis and correlation studies between gene expression and metabolite profiles.

Main Results

  • Significant differences in gene expression and metabolic profiles were observed between FTO KO and WT cells.
  • Enrichment analysis identified involvement in metabolic pathways, cellular functions, and disease-related cascades (e.g., atherosclerosis, insulin signaling).
  • Metabolomic profiling showed distinct metabolic alterations in FTO KO cells, with melatonin correlating with multiple gene expressions.

Conclusions

  • FTO gene deficiency profoundly impacts cellular gene expression and metabolism.
  • The study highlights FTO's involvement in diverse biological pathways, including metabolic and signaling processes.
  • Findings provide a basis for further research into FTO's role in physiological and pathological conditions.

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