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Updated: Jun 7, 2025

Defining Substrate Specificities for Lipase and Phospholipase Candidates
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Tailoring CotA Laccase Substrate Specificity by Rationally Reshaping Pocket Edge

Tian Xie1,2, Jiakun Li1,2,3, Ganggang Wang1,2

  • 1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, P. R. China.

Chembiochem : a European Journal of Chemical Biology
|November 16, 2024
PubMed
Summary

Mutations in bacterial multicopper oxidase CotA enhanced activity towards large substrates like ditaurobilirubin and significantly boosted expression yields in E. coli. These findings offer insights for enzyme engineering and industrial applications.

Keywords:
laccaseprotein engineeringprotein expressionspecific activities

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