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Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation
Published on: June 26, 2018
Protocol for generation of PD modeling induced neurons and detection of α-synuclein forms
Francesco V Nardi1, Gulimiheranmu Maisumu1, You Zhou2
1Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Boston, MA 02115, USA; Biomedical Engineering Program, College of Engineering and Mines, University of North Dakota, Grand Forks, ND 58202, USA.
Abstract:
Alpha-synuclein (α-Syn) is an important molecule in the pathogenesis of Parkinson's disease and Alzheimer's disease-related dementias such as Lewy body dementia, forming multiple pathological species. In vitro disease models, including human neurons and α-Syn-transfected cells, are instrumental to understand synucleinopathies or test new therapies. Here, we provide a detailed protocol to generate human neurons derived from induced pluripotent stem cells (iPSCs), and HEK cells, with α-Syn mutations. We also describe multiple assays to determine the various α-Syn forms.
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