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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 7, 2025

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

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FlowDiff: a simple, flow cytometry-based approach for performing a leukocyte differential count.

Konstantinos Dimopoulos1, Delphine Bonneau1, Jens Hannibal1,2

  • 1Department of Clinical Biochemistry, Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark.

Scandinavian Journal of Clinical and Laboratory Investigation
|November 16, 2024
PubMed
Summary
This summary is machine-generated.

FlowDiff, an 8-color flow cytometry panel, offers a rapid and accurate alternative to manual leukocyte differential counts. This method demonstrates high diagnostic accuracy, successfully identifying various hematologic malignancies.

Keywords:
Differential leukocyte countcluster of differentiation markerflow cytometryhematology

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Area of Science:

  • Hematology
  • Immunophenotyping
  • Clinical Pathology

Background:

  • Manual leukocyte differential counts are time-consuming and subjective.
  • Flow cytometry offers a more objective and efficient approach to cell analysis.

Purpose of the Study:

  • To develop and validate FlowDiff, an 8-color flow cytometry panel, as a replacement for manual leukocyte differential counts.
  • To assess the diagnostic accuracy of FlowDiff in identifying various leukocyte populations and hematologic malignancies.

Main Methods:

  • Development of a single-tube, 8-color flow cytometry panel (FlowDiff).
  • Utilized a stain-lyse no wash protocol (approx. 30 min working time).
  • Compared FlowDiff results with Sysmex XN analyzer and manual differentials in normal and abnormal samples.

Main Results:

  • Excellent correlation between FlowDiff and Sysmex XN in normal samples.
  • Very good correlation and high diagnostic accuracy with manual differentials in abnormal samples.
  • Accurate identification of acute leukemia and B-lymphomas; detection of previously undiagnosed B-lymphomas.

Conclusions:

  • FlowDiff is a reliable and accurate method for leukocyte differential counting.
  • FlowDiff can successfully substitute the manual differential count in clinical laboratories.
  • The 8-color flow cytometry panel offers improved efficiency and diagnostic capabilities.