Exploring divalent metal ion coordination. Unraveling binding modes in Staphylococcus aureus MntH fragments
Valentyn Dzyhovskyi1, Maurizio Remelli2, Kamila Stokowa-Sołtys3
1Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland.
Journal of Inorganic Biochemistry
|November 16, 2024
Summary
Staphylococcus aureus uses the MntH transporter to acquire essential divalent metal ions. This study reveals the N-terminal fragment of MntH shows high stability with copper(II) and iron(II) ions.
Area of Science:
- Biochemistry
- Microbiology
- Structural Biology
Background:
- Bacterial metal ion coordination is vital for metabolism and enzyme function.
- Staphylococcus aureus requires divalent metal ions for virulence and metabolic processes.
- The MntH transporter facilitates the uptake of essential divalent metal ions in S. aureus.
Purpose of the Study:
- To investigate the coordination modes of copper(II), iron(II), and zinc(II) with MntH fragments.
- To determine the metal-binding preferences and thermodynamic stability of MntH peptides.
- To compare MntH binding affinities with other bacterial transporters.
Main Methods:
- Potentiometry
- Mass spectrometry
- Spectroscopic methods
- Analysis of four model peptides representing MntH fragments.
Main Results:
- Established coordination modes for Cu(II), Fe(II), and Zn(II) with MntH peptide fragments.
- The N-terminal fragment of MntH exhibited the highest thermodynamic stability with Cu(II) and Fe(II) at physiological pH.
- Differential binding affinities were observed between MntH peptides and peptides from the S. aureus FeoB transporter.
Conclusions:
- The N-terminus of MntH plays a significant role in coordinating essential metal ions like copper and iron.
- Understanding MntH metal coordination provides insights into bacterial metal homeostasis and virulence.
- Comparative analysis highlights transporter-specific metal-binding characteristics.
Keywords:
Copper(II) complexesIron(II) complexesMetal-peptide complexesMntH transporterStaphylococcus aureusZinc(II) complexesMore Related Videos
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