Fasciolopsis buski isolated from human host, India has distinct clade based on nuclear ribosomal DNA sequences
Sandhya Chaurasiya1, Aradhana Singh1, Anurag Tiwari2
1Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Abstract:
Fasciolopsiasis caused by Fasciolopsis buski, is a significant cause of morbidity. In the present study F. buski detected during endoscopy of a 50-year-old male patient form Uttar Pradesh, India was used for phylogenetic analysis based on 28S rDNA and ITS2 regions. The parasite was genetically similar to an isolate of pig from Meghalaya. The intra-species genetic variation for 28S rDNA was 1-3% (India), 5% (Vietnam) and for ITS2, 1-2% (India) while 17 % from Vietnam. Thus, F. buski from humans or pigs from India were closely related than those from Vietnam and China which were grouped into separate clades.
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