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Related Experiment Video

Updated: Jun 7, 2025

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based

Axel Stoecker¹, Diana Pinkert-Leetsch², Timea Koch³

¹Faculty of Engineering and Health, University of Applied Science and Arts, 37085, Goettingen, Germany.

Scientific Reports

|November 16, 2024

Summary

This summary is machine-generated.

Three-dimensional light sheet fluorescence microscopy (LSFM) visualizes unlabeled corneal tissue stiffness changes. This label-free method aids in examining diseases like keratoconus by assessing altered tissue morphology and stiffness.

Keywords:

3D virtual histology Autofluorescence Corneal stroma Extracellular matrix stiffness Light sheet microscopy Tomographic microscopy

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Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

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Published on: January 6, 2018

Area of Science:

Ophthalmology
Biomedical Engineering
Biophysics

Background:

Stiffness-related eye diseases, such as keratoconus, involve complex changes in the corneal matrix.
Accurate visualization of these morphological changes is crucial for diagnosis and treatment.

Purpose of the Study:

To utilize three-dimensional (3D) light sheet fluorescence microscopy (LSFM) for label-free analysis of corneal tissue stiffness.
To qualitatively visualize morphological matrix changes in corneal tissue samples.

Main Methods:

Porcine corneal tissue samples were treated with NaCl or glutaraldehyde (GTA) to alter stiffness.
Samples were cleared using benzyl alcohol/benzyl benzoate (BABB) and scanned with LSFM.
LSFM data was validated using conventional paraffin embedding and planar microscopy.

Main Results:

LSFM successfully identified 2D/3D morphology of unlabeled corneal tissue via autofluorescence.
Glutaraldehyde treatment enhanced collagen crosslinking, inducing detectable morphological changes in autofluorescence signals.
LSFM findings correlated with conventional histology, confirming the method's validity.

Conclusions:

LSFM enables label-free, 3D autofluorescence assessment of corneal morphology within its anatomical context.
This technique provides a basis for examining pathologically altered corneas and related diseases based on tissue stiffness.
LSFM facilitates ophthalmologic examinations of corneal diseases characterized by altered tissue stiffness.