A Multimode fluorescent sensor for sequential detection of Cu²⁺ and cysteine as well as pH sensor with real sample...

Highly responsive and optically selective (E)-1-((4-phenoxyphenyl) diazenyl)naphthalen-2-ol) sensor PDN with aggregation induced emission enhancement (AIEE) properties has been developed for the sequential detection of Cu²⁺ and L- Cysteine through fluorescence On-Off-On strategy. The selectivity of sensor depends on the presence of a diazo functional group and its appropriate cavity location in sensor molecule. Azo dye-based (E)-1-((4-phenoxyphenyl) diazenyl)naphthalen-2-ol) sensor PDN has been synthesized by utilizing a simple diazotization synthetic methodology that showed extraordinary AIEE behavior with bathochromic shift owing to the formation of J-aggregates. The morphology and size of aggregates were analyzed by SEM and DLS analysis, respectively. The calculated LOD of sensor PDN for Cu²⁺, and L-cysteine is 0.113 nM, and 84 nM, respectively. Fluorescence, UV-visible, LC-MS, ¹H and ¹³C NMR titration were carried out to understand the interaction of sensor with Cu²⁺. The sensor was practically utilized in the sequential sensing of Cu²⁺ and Cys in real samples. Interestingly, sensor PDN was successfully employed for the sensing of a strong acid and base as well as the detection of Cu²⁺ ions in the solid state. Moreover, these experimental results were supported through DFT calculations.