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Related Experiment Video

Updated: Jun 7, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Simple generation of cleavable labels for multiplexed imaging.

Vincent Van Deuren¹, Silke Denis¹, Robin Van den Eynde¹

¹Laboratory for Nanobiology, Department of Chemistry, KU Leuven, Leuven, Belgium. vincent.vandeuren@kuleuven.be.

Chemical Communications (Cambridge, England)

|November 18, 2024

Summary

This summary is machine-generated.

This study introduces a simple, inexpensive method for amine-targeted antibody labeling using a cleavable linker. This technique facilitates efficient multiplexed immunohistochemistry and high-content imaging through easy label removal.

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Last Updated: Jun 7, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

Area of Science:

Biochemistry
Molecular Biology
Immunohistochemistry

Background:

Multiplexed immunohistochemistry commonly uses cyclic staining, imaging, and regeneration.
Existing methods can be complex and costly.

Purpose of the Study:

To develop a simple, inexpensive amine-targeted antibody labeling method.
To enable efficient cyclic staining-imaging-removal for multiplexed assays.

Main Methods:

Amine-targeted antibody labeling using a cleavable linker.
One-pot reaction with readily available reagents.
Demonstration on isolated cells and tissue sections.

Main Results:

Over 94% of labels removed in 30 minutes with mild reducing agent.
Up to 99% label removal achieved within 1 hour.
Method applicable to repeated staining-imaging-removal cycles.

Conclusions:

The developed method simplifies multiplexed immunohistochemistry and high-content imaging.
Introduces a convenient way to incorporate cleavable linkers.
Offers an accessible approach for researchers without synthetic experience.