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Fenugreek seed proteins: Ultrasonic-assisted extraction, characterization, and cupcake application
Izzet Turker1, Gamze Nur Olgun1, Hilal Isleroglu1
1Faculty of Engineering and Architecture, Food Engineering Department Tokat Gaziosmanpasa University Tokat Turkey.
Food Science & Nutrition
|November 18, 2024
Summary
Fenugreek seed protein extraction was optimized using ultrasonic-assisted methods, achieving a high yield. The resulting protein isolates demonstrated valuable functional, structural, and nutritional properties, showing potential for enhancing food products.
Area of Science:
- Food Science
- Biochemistry
- Chemical Engineering
Background:
- Fenugreek seeds are a rich source of protein with potential applications in food.
- Optimizing extraction methods is crucial for maximizing protein yield and functionality.
Purpose of the Study:
- To optimize ultrasonic-assisted extraction of fenugreek seed proteins.
- To characterize the functional, structural, and nutritional properties of the protein isolates.
- To evaluate the application of fenugreek protein isolates in bakery products.
Main Methods:
- Ultrasonic-assisted extraction with varying solid:solvent ratios and sonication amplitudes.
- Characterization of protein isolates including yield, functional properties (coagulation, water/oil holding, foaming, emulsion), SDS-PAGE, FT-IR, and amino acid analysis.
- Incorporation of protein isolates into cupcakes and assessment of baking quality.
Main Results:
- Optimal extraction yield of 98.74% achieved at specific solid:solvent ratio and amplitude.
- Protein isolates exhibited significant functional properties and a rich essential amino acid profile.
- Cupcakes enriched with fenugreek protein isolates showed acceptable quality attributes.
Conclusions:
- Ultrasonic-assisted extraction is effective for obtaining high-yield fenugreek seed protein isolates.
- Fenugreek protein isolates possess desirable characteristics for food applications.
- Fenugreek seeds can be utilized to improve the nutritional value of bakery products.
