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Light transmission aggregometry for platelet function testing: position paper on current recommendations and French
Alain Stépanian1, Florence Fischer2, Claire Flaujac3
1Service d'Hématologie Biologique, PhyMedExp UMR UM - CNRS 9214 - Inserm U1046, CHU de Montpellier, Université de Montpellier, Montpellier, France.
Platelets
|November 18, 2024
Summary
This study provides 28 proposals for accrediting light transmission aggregometry (LTA), a key test for platelet function disorders. These recommendations cover all assay aspects, aiming to improve LTA test standardization and reliability in specialized labs.
Area of Science:
- Hematology
- Clinical Pathology
- Laboratory Accreditation
Background:
- Light transmission aggregometry (LTA) is crucial for assessing platelet function, particularly in diagnosing platelet disorders.
- Existing national guidelines aid LTA test implementation but lack specific details for ISO 15189 accreditation.
- Sample matrix specificities in LTA require precise positioning for laboratory accreditation.
Purpose of the Study:
- To develop comprehensive recommendations for Light Transmission Aggregometry (LTA) accreditation according to ISO 15189.
- To address preanalytical, analytical, and postanalytical aspects of LTA testing.
- To provide a French position paper on LTA best practices and accreditation.
Main Methods:
- Review of existing national guidelines and recommendations for LTA testing.
- A 2021 survey of LTA practices among members of the Société Française de Thrombose et d'Hémostase (SFTH).
- Formulation and voting on 28 proposals for LTA accreditation by the SFTH.
Main Results:
- A total of 28 proposals covering all LTA assay aspects were approved by the SFTH.
- Recommendations address blood sampling, PRP preparation, instrumentation, agonists, performance evaluation, and data interpretation.
- The proposals provide a detailed framework for LTA accreditation, including preanalytical, analytical, and postanalytical considerations.
Conclusions:
- The developed proposals offer a robust framework for LTA accreditation, ensuring standardized and reliable platelet function testing.
- This French position paper provides valuable guidance for laboratories and auditors seeking LTA accreditation under ISO 15189.
- The recommendations aim to enhance the quality and consistency of LTA assays globally.
