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Related Experiment Video

Updated: Jun 7, 2025

Establishing a Mouse Model of Thin Endometrium
05:26

Establishing a Mouse Model of Thin Endometrium

Published on: November 1, 2024

559

Establishing a Mouse Model of Thin Endometrium.

Mingye Chen1, Junjie Tang2, Binfa Liang1

  • 1Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Institute for Reproductive Medicine and Genetics, Shenzhen Zhongshan Obstetrics and Gynecology Hospital (formerly Shenzhen Zhongshan Urology Hospital); Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri-Implantation.

Journal of Visualized Experiments : Jove
|November 18, 2024
PubMed
Summary

Researchers developed a reliable mouse model for thin endometrium (TE), a key cause of infertility. This new method uses uterine ethanol injection, significantly reducing mortality and adhesions, offering a platform for future TE treatments.

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Area of Science:

  • Reproductive Biology
  • Infertility Research
  • Animal Models

Background:

  • Thin endometrium (TE) is a significant factor contributing to infertility.
  • Current understanding of TE pathogenesis is limited, necessitating effective treatments.
  • Existing animal models for TE, particularly the ethanol-induced mouse model, face challenges like high mortality and intrauterine adhesions.

Purpose of the Study:

  • To establish a refined protocol for inducing thin endometrium (TE) in a mouse model.
  • To minimize mortality rates and intrauterine adhesions associated with TE induction.
  • To provide a reliable and reproducible model for studying TE and developing therapeutic strategies.

Main Methods:

  • A modified protocol involving the injection of 95% ethanol into the mouse uterus.
  • Varying ethanol infusion times (1-3 minutes) were tested to optimize the model.
  • Assessment of endometrial thickness, gland count, and fibrosis to confirm TE development.

Main Results:

  • Successful induction of thin endometrium (TE) in all tested mice.
  • Infusion times between 1-3 minutes yielded consistent TE.
  • Observed reductions in endometrial thickness and gland count, alongside increased endometrial fibrosis.
  • Significantly low mortality rates and minimal intrauterine adhesions were achieved.

Conclusions:

  • The refined ethanol-induced mouse model offers a reliable and reproducible method for studying thin endometrium (TE).
  • This model presents a valuable platform for investigating TE pathogenesis and evaluating potential treatments for infertility.
  • The protocol's low mortality and adhesion rates make it a practical tool for reproductive research.